cDNA synthesis protocols. Relative yields of cDNA, following 4 cDNA synthesis protocols (synthesized from mirVana-extracted RNA), were calculated from PCR signals obtained for high-abundance (green bars, β-actin), medium-abundance (red bars, TGF-β1), and low-abundance (blue bars, Foxp3) mRNA transcripts. This was investigated with RNA from PBL blasts (A) and from PBMC obtained directly from blood (B). Signals for AMV were set to 1. *P < .05. #P < .01. **P < .001.