Representative example of the LM technique for the isolation of mature ECs from the spleen. (A-B) The photomicrographs showing the ECs of the luminal surface of the splenic vein of patients 6 and 9, respectively, after immunohistochemical staining with anti-CD34 mAb and counterstaining with Harris hematoxylin. (C-D) Capillary of the splenic pulp immunostained with anti-CD34 mAb (as in panels A and B) before (C) and after (D) being laser microdissected and catapulted into the cap of a PCR tube. Original magnification for panels A, B, and C was 40× and for panel D, 10× using LD-Arcoplan objectives. The microdissection laser system is equipped on an inverted microscope (Axiovert 200; Zeiss) linked to a Sony DXC-309F 3CCD color video camera.