ATG-induced TF activation is not correlated with PS membrane exposure. (A) Time course of cell-associated PCA, PS exposure, and PI uptake after exposing ATG-loaded THP1 cells to NHP. PCA is presented as percent of maximum, whereas PS exposure and PI uptake are presented as percent positive cells (mean ± SD, n = 5-7). (B) Similar binding of (left) annexin V-FITC and (right) lactadherin-FITC to ATG-loaded and NHP-exposed THP1 cells. Histograms are representative of experiments with (top) low and (bottom) high PS exposure. (C) IgG- (○) or ATG-loaded THP1 cells (●) were exposed to NHP for 5 minutes at 37°C. Samples were analyzed for both PCA (clotting assay) and PS exposure (annexin V-FITC binding). The dashed line indicates the upper limit of PS exposure on IgG-loaded cells (n = 22). (D) Effect of C5, C7, and C9 antibodies on PS externalization on ATG-loaded THP1 cells exposed to NHP. Representative (left) histograms and (right) summary statistics indicating percent annexin V-positive cells are shown (mean ± SD, n = 3-7). (E) Coincubation with 20 µM 2-CA enhances PS externalization on ATG-loaded and NHP-exposed THP1 cells. (Left) Representative histograms and (right) summary statistics are shown (mean ± SD, n = 4). (F) 2-CA does not affect TF activation on ATG-loaded and NHP-exposed THP1 cells (mean ± SD, n = 6).