Inhibition of group A Pak proteins leads to decreased bone marrow engraftment and homing. (A) Engraftment of control and PID-transduced cells. WT LSK were transduced with MIEG3 (▪) or PID (♦) retrovirus and transplanted into lethally irradiated B6.SJL recipient mice. Percentage of engrafted, GFP+ cells in peripheral blood of recipient at indicated time points post-bone marrow transplantation. Data represent mean ± SEM (N = 10 recipients per group). P < .01 for all time points of 2 independent experiments. (B) Homing of control (black) and PID (gray) transduced cells. At 48 hours posttransduction GFP+ LSK were isolated using fluorescence-activated cell sorter. 2.5 × 105 GFP+ cells were transplanted into lethally irradiated B6.SJL recipient mice. The number of GFP+ LSK determined by flow cytometry that homed to the bone marrow at 12 hours and were retained 48 hours postinfusion was determined using cells collected from the femur, tibia, and iliac crest. Data represent equivalent numbers of live events per group per time point (mean ± SEM; N = 6 recipient mice per group per time point). One representative experiment is shown of 2 independent experiments. *P < .05 (t test).
