GFP-CBX2 binds to the p21 and p14ARF loci, which is reduced upon knockdown of CBX2. (A) CB CD34⁺ cells were transduced with GFP control or GFP-CBX2 vectors, cells were expanded in liquid culture for 12 days, and ChIP experiments were performed on the p14^ARF and p21 loci. (B-C) Experiment was performed as in panel A, but cells were cotransduced with pLKO.1 mCherry shRNA vectors against SCR or CBX2. The reduced mean fluorescence intensity of GFP confirmed efficient knockdown of CBX2 protein (B), which coincided with reduced CBX2 binding to p14^ARF and p21 loci (C). IgG, immunoglobulin G.