Modulation of histamine- and serotonin-induced EC proliferation and angiogenesis by 3TSR peptide. (A-D) Histamine- and serotonin-induced ³H thymidine incorporation, transwell and scratch assay migration, and tube formation on serum-starved HUVEC that had been pretreated for 10 minutes with 50 nM of 3TSR peptide (n = 4; experiments were repeated 3 times, A, B, and D. In C, n = 20 from 3 independent experiments). ***P < .001 vs control, Student t test. (E) Nu/Nu mice received intradermal injections of 1 × 10¹¹ pfu of AAV-TSR in 100 μl of HBSS or HBSS alone. Two weeks later, histamine or serotonin pellets were implanted immediately beneath the AAV-TSR (b,d) or HBSS (a,c) injection sites, and tissues were collected 10 days later. Data are representative of experiments performed on 8 mice per group.