CARD9 deficiency in a patient with systemic candidemia. (A) Surface expression of (left) Dectin-1 and (right) Dectin-2 in control and patient PMNs and monocytes was measured by flow cytometry. Dotted lines represent controls and solid lines patient cells. Isotype controls are shown in gray. Comparable amounts of dectins were detected on the surface of patient and control cells. (B) Western blot detection of C-type lectins (Dectin-1, Dectin-2, and Mincle) as well as Bcl-10 and CARD9 in control and patient neutrophils (indicated as C and P, respectively). GAPDH was used as a loading control. Results are representative for 3 independent experiments. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.