Chemotherapy induces a physiologically relevant response. (A) Weights of mice were monitored for 4 weeks after initial exposure to single-agent or combined chemotherapy. Weights were normalized to the weight of each mouse on day 1 of the experiment. (B-G) CBC analysis was performed before the initial exposure to chemotherapy and serially each week for 4 weeks. (B) White blood cell (WBC) count, (C) neutrophil (NE) count, (D) red blood cell (RBC) count, (E) hemoglobin (Hb), (F) hematocrit (HCT), and (G) platelet counts were monitored. (H) Total BM cellularity of an individual femur was calculated on day 8 following initial exposure to chemotherapy. The average cell number for the PBS cohort of each experiment was normalized to 1.0. LSK flow was performed on these samples to determine (I) lin⁻ BM cell percentage, (J) LSK frequency in the BM, and (K) total LSK per femur for the control and DA groups. (L) Cell cycle analysis of PBS- and DA-exposed BM cells gated on LSK (top row) or total BM cells (bottom row). *P < .05 as determined by the Student t test. NS, not significant.