Figure 6
Cell entry and nuclear colocalization of AAV vectors. The human hepatocyte cell line HHL5 was incubated with AAV vectors at an MOI of 105 in a tissue culture incubator and assayed at 1, 5, and 24 hours for virus nuclear colocalization on an ImageStream imaging flow cytometer. (A) Virus intracellular localization. Five hours after the time course started, virtually all virions were internalized into hepatocytes. (B) Colocalization of fluorescently labeled viral particles and nuclei, shown as percent of total viral particles. Results from 1 representative experiment. All experiments were repeated and analyzed at least twice.
