T-Rapa cell products have relatively equal numbers of genes that are up- or downregulated in expression relative to culture input CD4⁺ T cells. T-Rapa cell products were manufactured ex vivo (n = 6) and compared with the purified CD4⁺ T cells used to initiate the cultures (n = 6). RNA was purified from each paired sample, with further analysis by gene expression microarray. The heat map illustrates that genes were consistently differentially expressed, with 18.1% of genes (6147 of 34 051) being differentially expressed in T-Rapa products relative to input CD4 cells (P < .001); the number of upregulated genes in T-Rapa cells was relatively equal to the number of downregulated genes.