T-Rapa cells express a balanced Th2/Th1 cytokine phenotype. (A) T-Rapa cell clinical products were analyzed by intracellular flow cytometry for expression of GATA-3 (Th2 cell transcription factor), T-bet (Th1 cell transcription factor), and FoxP3 (TREG cell transcription factor) (left). The intraproduct ratio of GATA-3:T-bet in T-Rapa cells was >1:1 (*P < .05). (B) T-Rapa cell clinical products were evaluated at the time of DLI (day 12 of culture) and after an additional culture interval intended to evaluate effector function and differentiation plasticity (day 18 of culture). At each time point, the T-Rapa cells were costimulated, and the resultant supernatants were tested for cytokine content by Luminex (mean ± standard error of the mean; n = 40; *, increase from day 12 to day 18, P < .05). Values are expressed as pg/mL (1 × 106 cells per mL per 24 hours).
