BOEC characterization: type 2A VWD. (A-B) Data from patient VWD-13. (A) VWF was measured by ELISA in the total cell lysate (left) and cell culture supernatant (right) from BOECs isolated from a type 2A VWD patient in the absence and presence of PMA stimulation (mean ± SEM from 3 replicates). (B) VWF expression from control or PMA-stimulated BOECs was visualized by confocal IF microscopy (scale bars represent 20 µm). Boxes show 2× magnified image. *P < .05; ***P < .001.