Figure 1
NK1R-DCs are mature DCs that do not secrete IL-10. (A-B) Expression of Ag- presenting (MHC class II, IAb), activation (CD83), and costimulatory (CD80, CD86, and CD40) molecules by untreated DCs, Ag-DCs, NK1R-DCs, and Ag-NK1R-DCs, analyzed by flow cytometry. (A) Percentages of positive DCs, (B) intensity of expression of each marker, as MFI. Means ± 1 SD of 3 independent experiments are shown. (C) Secretion of IL-12p70 and IL-10 (analyzed by ELISA) by untreated DCs, NK1R-DCs, Ag-DCs, and Ag-NK1R-DCs (the latter cultured with or without LPS). Means ± 1 SD from 3 independent experiments are displayed. MFI, mean fluorescence intensity.
