In sDLN, secretion of IL-12 is limited to endogenous DCs and induction of CTL function requires recruitment of inflammatory DCs. (A) Percentages of CD11c⁺Ly6C⁺ inflammatory DCs (flow cytometry) in sDLN after skin immunization with untreated DCs, Ag-DCs, or Ag-NK1R-DCs. (B) Further characterization (flow cytometry) of CD11c⁺Ly6C⁺ DCs coexpressing TNF-α or iNOS in sDLNs, 2 days after administration of WT Ag-NK1R-DCs, transduced or not with RAd–IL-10, to WT or CCR2^KO mice. (C) Identification (flow cytometry) in sDLNs of the DC subsets secreting IL-12p40/70, 2 days after skin immunization of WT or CCR2^KO mice with WT untreated-DCs, Ag-DCs, or Ag-NK1R-DCs, the latter transduced or not with RAd–IL-10. (D) Flow cytometric analysis of IL-12p70 kinetics in sDLNs following subcutaneous administration of Ag-DCs or Ag-NK1R-DCs. (E) Comparative analysis of the CTL function (in vivo killing assays), 7 days after skin vaccination of WT or CCR2^KO mice with WT, Ag-DCs, NK1R-DCs, or Ag-NK1R-DCs. (A-C) Results from 1 representative of 3 independent experiments are shown. (D) Means ± 1 SD of 5 mice per experimental group are shown.