Marked reduction of MZ B cells, but not FO or B1 B cells, or other immune cells in B-cell–specific Notch2-deficient mice. (A) Marked reduction of MZ B cells but normal FO and B1 B populations in B-cell–specific Notch2-deficient mice. Upper panels: Splenocytes from CD19CreNotch2+/+ and CD19CreNotch2fl/fl mice were stained with antibodies to B220, CD21, and CD23. In B220+-gated cells, FO B cells (CD21+CD23hi) and MZ B cells (CD21hi CD23lo/−) are shown. The percentages indicate cells in the gated B220+ population. Lower panels: Peritoneal cells from CD19CreNotch2+/+ and CD19CreNotch2fl/fl mice were stained with antibodies to IgM and CD5. In the gated live population, B1 B cells (IgM+CD5+) are shown. The percentages indicate cells in the gated live population. (B) Bar graphs show the percentages of MZ and FO B cells in the gated splenic B220+ population, and B1 B cells in the gated live peritoneal population from the indicated mice. (C) Bar graphs show the percentages of total (Thy1.2+), CD4, and CD8 T cells in the gated splenic lymphoid population from the indicated mice. (D) Bar graphs show the percentages of NK (NK1.1+), monocytes (CD11b+F4/80+), neutrophils (CD11b+Ly6G+), dendritic cells (DC) (CD11b+CD11c+), and erythrocytes (Ter119+) in the gated live splenocytes and of mast cells (c-Kit+FcεR+) in the gated live peritoneal cells from the indicated mice. Data are obtained from 3 CD19CreNotch2+/+ and 4 CD19CreNotch2fl/fl mice.
