Expression of ICAM-1 in CLL and normal B cells upon lenalidomide treatment. (A) A total of 2 × 105 CD19-enriched CLL or normal B cells were cocultured with 2 × 105 monocytes isolated by CD14-specific MACS from PBMC of healthy individuals in 250 µL complete medium in 48-well plates for 2 days in the presence or absence of 10 µM lenalidomide. ICAM-1 (CD54) expression in CLL cells and B cells was quantified by flow cytometry before and after culture. Scatter plots including mean values and SEM of relative MFI obtained in 8 CLL samples and 3 HD B-cell samples by independently performed experiments are shown. Paired Student t test revealed a statistically significant difference between treated and untreated CLL cells (**P = .008). (B) Ratios of relative MFI values for ICAM-1 of treated versus untreated cells were calculated for each analyzed sample and are depicted as dot blots including mean values for CLL and HD B cells. Student t test revealed a statistically significant difference between the two cell types (**P = .005). rel., relative.
