Transcription of KSHV miRNAs. (A) Transcription of pre-miRNAs and other transgenes was represented as RT-qPCR Ct values from 2 transgenic mice from 2 independent lines or a wild-type (WT) mouse. BC3, a PEL cell line, was used as a positive control. (B) Transcription of pre-miRNAs was validated using Bioanalyzer. RT+ and RT− represent RT-PCR reactions and reactions without reverse transcriptase, respectively. (C) Expression of mature miRNAs was confirmed using the TaqMan assay. Amplified products were visualized via the LabChip system. BC3, a PEL cell line was used as a positive control and U6 was used as a positive control for TaqMan assay system.