LPS-induced B-cell expansion in KSHV latency locus transgenic mice. (A-B) Frozen spleen sections were stained with B220 (green) and PNA (orange). Magnification ×200. KSHV latency transgenic mice were injected with LPS. On d 1 and 7 after injection, splenic cells were analyzed by fluorescence-activated cell sorter. (C-D) Percentages of activated GC B cells (CD19⁺CD71⁺PNA^hi) and activated MZ B cells (CD19⁺CD21^hiCD23^-CD69⁺) are shown on d 1 after LPS injection. Averages of 6 wild-type (WT) and 6 latency transgenic (TG/Tg) mice are indicated with the standard deviation. (E) Expression of CD69, an activation marker on the CD19⁺CD21hiCD23^- gated MZ B cells. Veh, vehicle.