CD56⁺LFA-1^- ILC22 cells do not become cNK cells. (A) At day 21, stage III progenitors (CD56⁺CD94^-CD117^high) were sorted on the basis of LFA-1 expression for ILC22 cells (LFA-1⁻) and cNK cells (LFA-1⁺). (B) Cells were cultured with IL-15, IL-7, SCF, and FLT3L or IL-15 alone for additional 7 days. Greater than half of the stage III cells that expressed LFA-1 at the time of sorting acquired CD94 and therefore differentiated into stage IV or V cNK cells. In contrast, stage III cells that were LFA-1⁻ did not acquire CD94 and maintained an ILC22 phenotype in either cytokine condition. (C) LFA-1⁻ stage III cells, but not LFA-1⁺ cells, produce IL-22 in response to IL-1β+23 after 7 days of culture in IL-15. In contrasts, the LFA-1⁺ cells express IFN-γ in response to IL-12+18. (D) After cultivation with IL-15 for 14 days, most of LFA-1⁺ sorted cells acquired CD94, whereas LFA-1⁻ stage III maintained LFA-1⁻CD94⁻ phenotype. Results are representative of 5 donors. D, day.