American Society of Hematology

Figure 2

$Figure 2. Glucose accelerates the onset and output of definitive hematopoiesis in the AGM. (A) Time-course analysis by in situ hybridization for runx1 in 3-hour intervals from 18 to 36 hpf revealed earlier and enhanced expression after glucose exposure (n ≥ 35/tx). (B) Electron microscopy of sagittal sections of embryos at 36 hpf revealed increased budding (arrowheads) of hemogenic endothelial cells (HSCs) from the ventral wall of the dorsal aorta (magnification: ×1200, top panels; ×10 000, bottom panels). (C) Transplantation of AGM cells from glucose-exposed CD41:eGFP embryos into irradiated adult WT recipients led to increased engraftment, as indicated by the fraction of recipients containing GFP+ cells by fluorescence microscopy 3 weeks after transplantation (t test, *P < .05, n = 28-31).$

Glucose accelerates the onset and output of definitive hematopoiesis in the AGM. (A) Time-course analysis by in situ hybridization for runx1 in 3-hour intervals from 18 to 36 hpf revealed earlier and enhanced expression after glucose exposure (n ≥ 35/tx). (B) Electron microscopy of sagittal sections of embryos at 36 hpf revealed increased budding (arrowheads) of hemogenic endothelial cells (HSCs) from the ventral wall of the dorsal aorta (magnification: ×1200, top panels; ×10 000, bottom panels). (C) Transplantation of AGM cells from glucose-exposed CD41:eGFP embryos into irradiated adult WT recipients led to increased engraftment, as indicated by the fraction of recipients containing GFP⁺ cells by fluorescence microscopy 3 weeks after transplantation (t test, *P < .05, n = 28-31).

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