Effect of group D and E anti-A2 mAbs on thrombin-catalyzed proteolytic cleavage of full-length fVIII. fVIII (100 nM) was incubated with 0.5 nM thrombin for 10 minutes at 37°C in the presence of the indicated mAb concentrations. Proteolytic cleavages were analyzed by SDS-PAGE as described in “Effect of anti-A2 mAbs on thrombin-catalyzed proteolytic cleavage of fVIII.” Ctrl, fVIII control not exposed to thrombin or anti-A2 mAbs; Stds, Molecular weight standards are 250, 150, 100, 75, 50, 37, and 25 kDa.