Effect of group D and E anti-A2 mAbs on thrombin-catalyzed proteolytic cleavage of the full-length fVIII/VWF complex. fVIII (100 nM) was incubated for 10 minutes at 37°C with VWF (1000 nM monomer equivalents) and then incubated for an additional 10 minutes in the presence of increasing mAb concentrations. Thrombin (0.5 nM) was added for 10 minutes and the reaction products were analyzed by SDS-PAGE as described in “Effect of anti-A2 mAbs on thrombin-catalyzed proteolytic cleavage of fVIII.” Ctrl, fVIII control not exposed to thrombin or anti-A2 mAbs; Stds, Molecular weight standards are 250, 150, 100, 75, 50, 37, and 25 kDa.