Macrophage Wnts suppress wound repair and angiogenesis. (A) Time-course of wound area in control and homozygous null Wls animals. (B) Images of wounds 2 and 5 days after initial injury (×20). (C) Quantification of wound area at day 5. (D) Percentage of F4/80-positive cells (macrophages) in control and mutant wounds. (E) Immunolabeling of wound sections for vasculature (PECAM) in control and null animals (×100). (F) Quantification of PECAM staining intensity per unit area in control and null wounds that was normalized to adjacent avascular epidermis. (G) Images of ARAs after 10 days of culture stained with isolectin-B4 (×50). (H) Quantification of ARA vessels in Wls control and mutant aortas. Statistical analysis performed was Student t test (A, D, F) and one-way analysis of variance with Tukey’s post-hoc test (C, H) using SPSS (IBM, Armonk, NY) software.