KIR2DS1-induced uptake of CCR7 in NK cells interacting with C2⁺ T-cell blasts or mDCs. (A) Two representative KIR2DS1⁺ NKG2A⁺ NK-cell clones (derived from 2 different C1/C1 donors) and 2 KIR2DS1⁻ NKG2A⁺ (from the same donors) were incubated at 37°C with CCR7⁺ allogeneic T-cell blasts derived from a C2/C2 donor. Co-incubation was performed in the absence or presence of specific anti–HLA-I (6A4) or anti-NKG2A mAbs. After 15 minutes, NK cells were harvested and stained with anti-CD56 and anti-CCR7 mAbs for cytofluorimetric analysis. The percentages of CCR7⁺ cells are indicated. Histogram plots (lower panel) illustrate the expression of CCR7 and HLA-E on T-cell blasts (median values are indicated). (B) The average of 6 independent experiments of KIR2DS1⁺ NKG2A⁺ or KIR2DS1⁻ NKG2A⁺ NK-cell clones incubated alone (NK) or with T-cell blasts is shown (left panel). NK clones (6 for each type) were derived from 3 different C1/C1 donors. The average of 6 independent experiments of KIR2DS1⁺ NKG2A⁺ NK-cell clones incubated with T-cell blasts, performed either in the absence or presence of specific anti–HLA-I (6A4) and anti-NKG2A mAbs, is shown (right panel). The histograms refer to the percentage of CCR7⁺ NK cells detectable after incubation with T-cell blasts under the indicated conditions. (C) Two representative KIR2DS1⁺ NKG2A⁺ NK-cell clones (derived from 2 different C1/C1 donors) and 2 KIR2DS1⁻ NKG2A⁺ clones (from the same donors) were incubated at 37°C in the absence or presence of anti–HLA-I (6A4) mAbs with allogeneic mDCs derived from a C2/C2 donor. After 15 minutes, NK cells were harvested and stained with anti-CD56 and anti-CCR7 mAbs for cytofluorimetric analysis. Percentages of CCR7⁺ cells are indicated. The histogram plots (lower panel) illustrate the expression of CCR7 and HLA-E on mDCs (median values are indicated). (D) The average of 6 independent experiments of KIR2DS1⁺ NKG2A⁺ or KIR2DS1⁻ NKG2A⁺ NK-cell clones incubated alone (NK) or with mDCs is shown (left panel). NK clones (6 for each type) were derived from 3 different C1/C1 donors. The average of 6 independent experiments of KIR2DS1⁺ NKG2A⁺ NK-cell clones incubated with mDCs in the absence or presence of specific anti–HLA-I (6A4) or anti-NKG2A mAbs is shown (right panel). The histograms indicate the percentage of CCR7⁺ NK cells under the indicated conditions. *P < .05; **P < .01; ***P < .001.