Mi2β positively regulates the expression of KLF1 and BCL11A in human CD34⁺ hematopoietic progenitor-derived primary erythroid cells. (A) qPCR analysis showing mRNA levels of KLF1 and BCL11A following Mi2β knockdown are decreased by 70% and 40%, respectively. (B) mRNA levels of KLF1 and BCL11A following MBD2 knockdown are not affected. (C) Western blot showing a decrease in the levels of BCL11A and KLF1 protein after Mi2β knockdown in human primary erythroid cells. (D) Western blot showing the level of MBD2 protein knockdown in human primary erythroid cells. (E) ChIP assay showing significant enrichment of Mi2β at the BCL11A and KLF1 promoter regions. Glyceraldehyde-3-phosphate dehydrogenase was used as a negative control, and enrichment values are normalized to IgG controls. Error bars represent the standard deviation of ≥3 independent experiments. *P < .05, **P < .02, and ***P < .001 according to the Students t test. NS = not statistically significant.