Ikaros partly inhibits Notch-mediated megakaryocyte differentiation. LSK and CMP cells purified by flow cytometry from wild-type C57BL/6 mice were transduced with an empty retroviral vector (MIE) or encoding full-length Ikaros encoding (IK1) and plated directly on OP9 and OP9-DL1 stroma for 5 and 7 days, respectively. A representative flow cytometry analysis is shown and lower panel bar graphs depict the means ± SD for 3 independent experiments. *P = .04; #P = .007.