Components of the Notch pathway are present on human moDCs and CD8⁺ T cells. (A) Relative expression of the five Notch ligands was determined by qRT-PCR in moDCs stimulated with LPS (100 ng/mL) for the indicated times. Samples were analyzed in triplicate. Shown is a representative example of 10 different donors. (B) Membrane expression of Notch1 and Notch2 was determined on CD8⁺ T cells by flow cytometry. PBLs were cultured with or without αCD3/CD28 microbeads for 4 days in the presence of IL-2 (30 IU/mL). The histograms shown represent live CD8⁺ T cells. Solid histogram, isotype control; black line, resting CD8⁺ T cells; gray line, activated CD8⁺ T cells.