Mks form podosomes on different substrata. Cells were spread for 3 hours on 100 μg/mL fibrinogen or fibronectin-coated surfaces or on glass, and then fixed and stained for F-actin (red, phalloidin) and WASp (A, C, D) or vinculin (B) (green). The right panel of pictures shows the merge of both channels. Mks were spread for 3 hours on 100 μg/mL Horm collagen-coated surface ±250 ng/mL SDF. Cells were analyzed for (E) the number of podosomes, (F) cell surface area, and (G) podosomes per μm². Scale bars represent 10 μm. Red square indicates enlarged area to highlight podosomes. Data are representative of at least 3 experiments.