MYD88 AS-PCR sensitivity assay. Serial dilutions of mutated and wild-type alleles (from 100% to 0% mutated alleles) were performed to identify the analytical sensitivity of AS-PCR. (A) Sensitivity of the method was ≥0.1%. (B) Amplification of the wild-type allele. All samples give rise to the wild-type MYD88 band, except for the 100% mutant allele sample.