Figure 5
Figure 5. Analyses of TCR and IgH gene rearrangements in iPSC lines. iPSC lines were tested for TCRβ, TCRγ, and IgH recombination by genomic DNA PCR using InvivoScribe Clonality Assays. Eight independent iPSC lines from 7 different donors (lanes 1-8) are shown: iNC-01 (lane 1), iGP91-03-1 (lane 2), iGP91-03-2 (lane 3), iGP91-04 (lane 4), iGP91-05 (lane 5), iGP91-06 (lane 6), iSCID-02 (lane 7), and iSCID-03 (lane 8). InvivoScribe Clonality Assay controls are shown as polyclonal (lane 9) and 2 different clonal controls (lanes 10 and 11) for each set. A 100-bp DNA ladder is also shown (lane 12). PCR products within the valid range of 69 to 129 bp for IgH, 80 to 220 bp for TCRγ, and 170 to 325 bp for TCRβ are shown. The arrow indicates the 100-bp size marker. The data shown are representative of 22 iPSC lines from 17 different donors.

Analyses of TCR and IgH gene rearrangements in iPSC lines. iPSC lines were tested for TCRβ, TCRγ, and IgH recombination by genomic DNA PCR using InvivoScribe Clonality Assays. Eight independent iPSC lines from 7 different donors (lanes 1-8) are shown: iNC-01 (lane 1), iGP91-03-1 (lane 2), iGP91-03-2 (lane 3), iGP91-04 (lane 4), iGP91-05 (lane 5), iGP91-06 (lane 6), iSCID-02 (lane 7), and iSCID-03 (lane 8). InvivoScribe Clonality Assay controls are shown as polyclonal (lane 9) and 2 different clonal controls (lanes 10 and 11) for each set. A 100-bp DNA ladder is also shown (lane 12). PCR products within the valid range of 69 to 129 bp for IgH, 80 to 220 bp for TCRγ, and 170 to 325 bp for TCRβ are shown. The arrow indicates the 100-bp size marker. The data shown are representative of 22 iPSC lines from 17 different donors.

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