Hemostatic effect of recombinant FVIII variant protein in HA mice. (A) The graph shows the time of full occlusion of the carotid artery upon 7.5% FeCl₃–induced injury. HA mice (C57BL/6) received 20 μg/kg of FVIII-RH (n = 5), FVIII-BDD (n = 4), or phosphate-buffered saline (PBS) (n = 3). (B) Blood loss following tail clipping is shown in the graph. HA mice (C57BL6/129Sv) received tail vein injection of FVIII-RH or FVIII-BDD at doses indicated or PBS (n = 5 mice per group). Hemostatically normal mice (n = 3, C57BL/6) were used as normal controls. Total blood loss (μL) was measured after tail transection at a 3-mm diameter, and blood was collected for 10 minutes. n.s., not significant.