Flow cytometry analysis of stress erythropoiesis in the spleen. (A) Gating strategy of Ter119 and CD71-stained splenocytes to define the EryA, EryB, and EryC populations.³³  The most immature cells are in the EryA population (CD71⁺, forward scatter (FSC)^high). (B) Examples of flow cytometry plots with percentages of EryA, EryB, and EryC populations indicated. (C) EryA populations in the spleens of mice with the four different genotypes. *P < .05 between mutant and control groups.