Figure 3
![Figure 3. PRT/H Abs activate platelets in a protamine-dependent manner. Washed 14C-serotonin–labeled platelets from healthy donors were incubated with or without protamine (PRT; 1.25 ug/mL) in the presence of 3 representative patient samples obtained at day 30 (CPB patients or control patients [CTRL]) with increasing amounts of unfractionated heparin (0, 0.25, 1, 100 U or 1U+ IV.3). 14C releasate was measured by liquid scintillation counting. These results are representative of 4 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/121/15/10.1182_blood-2012-11-469130/4/2828f3.jpeg?Expires=1735543601&Signature=shrBjcwig4q2u9rOppHaKtpM0DaVs1zELD~3-JL~I8CNKcxCleavVhdCT4aPozXnRo9cCZ6U9GbdEdBTf0WeWPoxMY4wQ8csFE8a-oOP9YkIbxrw9JDRphZcMHyib01qJDz6sKbT~ph9qZbOUYebyLJ9JKbKu0wRK2IQD67tSEdp9VoJnjSKFj6Y~-At4Lex0v-iJ0Xwe1phNDktRmQp2bKOL9Z1P-Seo4vjvODXl4Z41hTXRVmUjblpzvaXfAsvj4-Qflt-n4y3oBrzi8Bz0YGcSv5YyBN8DTdoQBKCz6x-4A7RHiv0twdvzZ-8~8OUEAEfDIrgrVnQFAkKLkAV0A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
PRT/H Abs activate platelets in a protamine-dependent manner. Washed 14C-serotonin–labeled platelets from healthy donors were incubated with or without protamine (PRT; 1.25 ug/mL) in the presence of 3 representative patient samples obtained at day 30 (CPB patients or control patients [CTRL]) with increasing amounts of unfractionated heparin (0, 0.25, 1, 100 U or 1U+ IV.3). 14C releasate was measured by liquid scintillation counting. These results are representative of 4 independent experiments.
