Expression levels of the NPM1 mutation A in mouse hematopoietic tissues. (A) RT-PCR detected transcription of the NPM1 mutant (NPMc⁺) mRNA in the BM of mutant mice 2 months after pIpC induction of the Cre. (B) Expression of the NPM1 mutant protein in peripheral blood lysates (left) and in hematopoietic tissues (right) using a specific antibody against the mutant. (C) Different expression levels of the NPM1 mutant in BM cells from heterozygous (Npm1-TCTG/WT;Cre⁺) vs homozygous (Npm1-TCTG/TCTG;Cre⁺) mice normalized to endogenous Npm1. (D) Western blot of magnetic-activated cell sorting–sorted CD41⁺ megakaryocytic (Mk), Gr-1⁺ myeloid (My), and CD3/B220⁺ T- and B-lymphoid (Ly) cells compared with whole BM of Npm1-TCTG/WT;Cre⁺ pIpC-induced mice. (E) NPM1 mutant protein expression in 3 different NPM1-mutated AML patients (P1, P2, and P3) compared with heterozygous (HET) and homozygous (HOMO) mutant mice BM. (F) Western blot analysis of NPMc⁺ expression on nuclear and cytoplasmic fractions of BM cells from heterozygous and homozygous mutant mice. C, cytoplasmic fraction; N, nuclear fraction; TL, total lysate.