miR-10a, miR10b, and miR20a overexpression correlates with HOXB gene expression. (A) miRNA qRT-PCR for miR-10a, miR-10b, and miR-20a in CD41⁺ cells from Npm1-TCTG/WT;Cre⁺ and Npm1-TCTG/TCTG;Cre⁺ mice vs Cre⁻ controls. (B) Relative gene expression of HoxB4 and HoxB5 in the BM of Npm1-TCTG/WT;Cre⁺ and Npm1-TCTG/TCTG;Cre⁺ mice vs Cre⁻ controls. qRT-PCR was performed on pooled CD41⁺ magnetic-activated cell sorting–sorted cells from ≥3 mice per genotype. Histograms represent mean miRNA expression from triplicate samples obtained from 3 independent experiments. *P < .05, **P < .01, ***P < .001 (unequal-variance t test).