Transcriptional alterations of HSC aging persist through transplantation-mediated stress. (A) Experimental outline. (B) Venn diagrams depicting the number of genes up-regulated or down-regulated in HSCs with age, and the overlap of genes that remain differentially regulated as a consequence of HSC aging after transplantation. Numbers in Venn diagrams depict differentially regulated probe sets, with numbers in brackets indicating unique genes. The identities of common genes are shown below each diagram. The analysis was performed on gene expression arrays from 2 independent experiments totaling 6 replicate arrays for the young and aged steady-state setting and 2 replicate arrays for the young and aged transfer setting.