Figure 3
Figure 3. Single-cell telomere length analysis of HSCs from various sources. (A) The relative telomere length was measured by qPCR in single sorted HSCs in 2 independent experiments from 3 individual young and aged mice (n = 251 and 246 cells in each group, respectively). (B) Single cell telomere length measurements of blastocyst- and agedBiPS2-derived HSCs from an agedBiPS2 primary chimera (n = 86 and 66 cells in each group, respectively). (C) Relative telomere length in blastocyst, agedBiPS1- and agedBiPS2-derived HSCs isolated from repopulated recipient mice (2 independent isolations, n = 200, 130, and 53 cells, respectively). (D) Relative telomere length in (left) blastocyst and agedBiPS derived HSCs from a germline founder (agedBiPS2 primary chimera, n = 43 and 41 cells in each group, respectively) and (right) from a germline offspring mouse compared with an age-matched C57BL/6 control (n = 84 cells in each group). Error bars indicate mean ± SEM.

Single-cell telomere length analysis of HSCs from various sources. (A) The relative telomere length was measured by qPCR in single sorted HSCs in 2 independent experiments from 3 individual young and aged mice (n = 251 and 246 cells in each group, respectively). (B) Single cell telomere length measurements of blastocyst- and agedBiPS2-derived HSCs from an agedBiPS2 primary chimera (n = 86 and 66 cells in each group, respectively). (C) Relative telomere length in blastocyst, agedBiPS1- and agedBiPS2-derived HSCs isolated from repopulated recipient mice (2 independent isolations, n = 200, 130, and 53 cells, respectively). (D) Relative telomere length in (left) blastocyst and agedBiPS derived HSCs from a germline founder (agedBiPS2 primary chimera, n = 43 and 41 cells in each group, respectively) and (right) from a germline offspring mouse compared with an age-matched C57BL/6 control (n = 84 cells in each group). Error bars indicate mean ± SEM.

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