SNAP-23 phosphorylation in stimulated platelets. (A) Mouse platelets were stimulated with the indicated agonists (thrombin, 0.1 U/mL; A23187, 1 μM; collagen, 10 μg/mL; PMA, 200 nM) for 3 minutes and were subjected to immunoblotting with anti-phospho-Ser95 (pSer-95) and anti-SNAP-23 antibodies. (B) Mouse platelets were preincubated with increasing concentrations of IKK inhibitors (TPCA-1, 0.5, 1 μM; BMS-345541, 5, 10 μM; BAY 11-7082, 12.5, 25 μM) for 5 minutes, stimulated with thrombin (0.1 U/mL; 3 min), and extracts from them were subjected to immunoblotting with pSer-95 and anti-SNAP-23 antibodies.