Figure 2
![Figure 2. IKK enzyme is active and the upstream signaling CBM complex is present in platelets. (A) Mouse and human platelets were held resting (R) or stimulated with thrombin (0.1 U/mL, 3 minutes; S) and extracts from them were subjected to immunoblotting with anti-CARMA1, anti-MALT1, anti-Bcl10, anti-IKK-α, anti-IKK-β, and anti-IKK-γ/NEMO antibodies. (B-C) Mouse platelets were preincubated with IKK inhibitors (BMS-345541, 5 μM; TPCA-1, 0.5 μM [B]) or BAY 11-7082, 12.5 μM; [C]) for 5 minutes and stimulated with thrombin prior to immunoblotting the extracts with anti-pSer32-IκB (pIκB) or anti-IκB antibodies.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/121/22/10.1182_blood-2012-11-470468/5/4567f2.jpeg?Expires=1735814418&Signature=06Xv5~H6CJtVCFKAraW7GR6FV52FfalGz-v1Lc4UxKF0PpkEcemhzc0hTg9ChR5DuXYPKEQjsWvdkYeyfesIJzz5sPVcasluZizdD4KuSgXn-vcq3RKIifH2AQosXkOyXDB72Pc94RjpxTIuKThhkQB8WsT7wHVSXmNe8d9uxrfEx4Jilu20FX4L~nL6pcL7Lo2LS317DbjdxexDBNYDJK1mJpYyM6HB7IlvKDpOz9PY9aMkBABlZ0FF4NO-3wz~PskWWcDxf4HuHccyikn6K~0xFoEu75ldvtAM1uh2gHj0p5cAbOJQ95tWkntHrmZV~6HUuw8l8I6~Oh~GPO~edg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IKK enzyme is active and the upstream signaling CBM complex is present in platelets. (A) Mouse and human platelets were held resting (R) or stimulated with thrombin (0.1 U/mL, 3 minutes; S) and extracts from them were subjected to immunoblotting with anti-CARMA1, anti-MALT1, anti-Bcl10, anti-IKK-α, anti-IKK-β, and anti-IKK-γ/NEMO antibodies. (B-C) Mouse platelets were preincubated with IKK inhibitors (BMS-345541, 5 μM; TPCA-1, 0.5 μM [B]) or BAY 11-7082, 12.5 μM; [C]) for 5 minutes and stimulated with thrombin prior to immunoblotting the extracts with anti-pSer32-IκB (pIκB) or anti-IκB antibodies.
