Bleeding time measurements in BMS-345541-treated and platelet-specific IKKβ^−/− mice. (A) Mice were given either 3% Tween 80 (5 mL/kg, vehicle) or 10 mg/kg BMS-345541 in 3% Tween 80 by oral gavage. Two hours post-dosing, tail bleeding time measurements were made as described in our “Methods” section. (B) PF4-Cre⁻ littermates (WT) or platelet-specific IKK-β deletion (IKK-β^−/−) mice were subjected to tail bleeding measurements. Each point represents the bleeding time of a single animal. Statistical significance was determined by unpaired t test using Sigma Plot software (Systat Software, Inc., San Jose, CA).