IL-12Rβ1–deficient subjects altered GC responses. Alteration of GC formation in IL-12Rβ1–deficient subjects. Immunohistochemistry staining of peripheral LN samples from IL-12Rβ1–deficient subjects and tonsil samples from control subjects. Samples were stained with (A) H&E, (B) CD20 mAb, (C) CD3 polyclonal Ab, (D) CD4 mAb, (E) CD57 mAb, (F) Bcl-6 mAb, and (G) AID mAb. T-B cell aggregates in subjects 1 and 2 are indicated by dotted lines. In control tonsils, secondary follicles are indicated by dotted lines, and the solid lines indicate the border between the mantle zone (MZ) and the GC. Bar equals 100 μm. (H) CD57⁺ cells per T-B cell aggregates. CD57⁺ cells were counted within the T-B cell aggregates in subject 2 LNs, and within GCs with a similar size present in tonsils from 3 control subjects. Student t test.