Expression of endogenous RUNX1a and RUNX1b/c parallels lineage commitment and hematopoietic emergence from hPSCs. (A) RT-qPCR analysis of endogenous RUNX1a throughout EB development from hESC (H9) or hiPSC (iCB5). GAPDH is used as an internal control. Results from day 13 EBs are set as 1. Bar chart represents relative RUNX1a expression at various time points. Error bars represent standard deviations (SDs) of 3 independent experiments. (B) RT-qPCR analysis of endogenous RUNX1b/c throughout EB development from hESC (H9) or hiPSC (iCB5). GAPDH is used as an internal control. Results from day 13 EBs are set as 1. Bar chart represents relative RUNX1b/c expression at various time points. Error bars represent SDs of 3 independent experiments. (C) RT-qPCR analysis of endogenous OCT4 and NANOG throughout EB development from hESCs (H9). GAPDH is used as an internal control. Results from undifferentiated cells (D0) are set as 1. Bar chart represents relative OCT4 and NANOG expression at various time points. Error bars represent SDs of 3 independent experiments. (D) Developmental progression of hemato-endothelial and hematopoietic surface marker expressions on disaggregated H9 cells at various time points. y-axis represents percentage of cells with positive staining in FACS analysis. Each time point represents the mean of 3 to 5 independent experiments.