Efficient and enhanced mesoderm and hematopoietic differentiation on RUNX1a overexpression in hESC (H9) cells. (A) Confocal fluorescence images of day 6 EBs derived from RUNX1a-transduced H9 cells show expression of GFP, CD31, and CD34. Scale bars, 200 µm. (B) Representative FACS analysis of CD31/CD34 expression on day 7 EBs derived from vector or RUNX1a transduced H9 cells. (C) Summary of FACS analysis from panel B. Percentages of 3 populations are shown: CD31⁺CD34⁻, CD31⁺CD34⁺, and CD31⁻CD34⁺. Error bars represent SDs of 3 independent experiments.*P = .0001, **P = .02.