Reconstitution of NSG mice engraftment. Approximately 1.5 × 105 to 3 × 105 RUNX1a progenitor cells (CD34+CD45+) were injected into the femur of irradiated NSG mice (n = 15). (Ai) Representative histogram analysis of human CD45 (hCD45) expression in recipient mice 9 weeks after transplantation. (Aii) Comparative analysis of hCD45 expression level as human chimerism in injected femur and contralateral femur in recipient mice (n = 5). (B) hCD45+ cells in recipient bone marrow 9 weeks after transplantation were measured and analyzed by flow cytometry. hCD45+ cells were further gated and analyzed for myeloid (hCD33+) and B-lymphoid (hCD19+) populations. MHC-I+ was gated for erythroid cells (CD36+) compositions. (Bi) Representative results from FACS analysis. (Bii) Summary of FACS analysis on 5 surviving recipient mice. (C) Representative RT-qPCR analysis of human RUNX1a in bone marrow of recipient mice after 6 weeks of survival.
