Comparison of HIV-2 Gag-specific responses. Comparison of HIV-2 Gag-specific CD8⁺ (A) and CD4⁺ (B) IFN-γ responses in HIV-2 controllers (n = 33) and viremic subjects (n = 27) (2-tailed Mann-Whitney U test). Comparison of polyfunctional (3- or 4-function) Gag-specific CD4⁺ T-cell responses in HIV-controllers (n = 33) and viremic subjects (n = 27) is displayed as percentage of polyfunctional Gag-specific CD4⁺ T-cells (C) and proportion of the total Gag-specific CD4⁺ response that is polyfunctional (D) (2-tailed Mann-Whitney U test). Bars represent medians. NS, not significant.