HIV-2 viral control is associated with polyfunctionality of the Gag-specific CD8⁺ T-cell response. (A) Proportion of response made up by different factors in HIV-2 controllers (VL < 100) and viremic subjects (VL ≥ 100). (B) Magnitude of polyfunctional Gag-specific CD8⁺ T cells in HIV-2 controllers and viremic subjects (2-tailed Mann-Whitney U test). (C) Inverse correlation between CD8⁺ polyfunctionality and HIV-2 VL. (D) Direct correlation between CD8⁺ monofunctionality and HIV-2 VL (Spearman’s rank correlation coefficient). All comparisons included n = 33 HIV-2 controllers and n = 27 viremic subjects. NS, not significant.