HIV-2–specific CD8⁺T cells display an earlier differentiated phenotype than CMV-specific CD8⁺T cells. CD27 (A) and CD27/CD45RO (B) coexpression according to viral specificity. ***P < .001; ** P < .01; *P < .05 (Kruskal-Wallis test with Dunn’s post test for multiple comparisons). HIV-2–specific CD8⁺ populations (n = 25) are derived from 21 HIV-2 subjects (n = 16 controllers, n = 5 viremic donors). CD27% expression on cells from viremic donors is highlighted in red in panel A. (C) Examples of CD27/CD45RO distribution of HIV-2–, CMV-, and EBV-specific CD8⁺ T cells, with percentage distribution in each quadrant displayed.