HIV-2–specific CD8⁺T-cells show high levels of PD-1, 2B4, and CD160 coexpression despite low VLs. (A) Higher expression of inhibitory receptors on HIV-2–specific CD8⁺ T cells when compared with intrapatient bulk CD8⁺ populations (n = 25 tetramer-positive populations from 21 HIV-2 subjects: 16 controllers and 5 viremic donors; 2-tailed Wilcoxon matched pairs test). Values from viremic donors are highlighted in red. (B) Comparison of PD-1⁺2B4⁺CD160⁺ coexpression on different virus-specific CD8⁺ T cells showing significantly lower coexpression on CMV-specific CD8⁺ T cells when compared with HIV-2–specific or EBV-specific CD8⁺ T cells. *P < .05 in a Kruskal-Wallis test with Dunn’s post test for multiple comparisons. Values from viremic donors are highlighted in red. (C) Example of inhibitory receptor expression on total CD8⁺, HIV-2–specific, and CMV-specific CD8⁺ T cells from a single donor. Red dots represent cells coexpressing CD160 in addition to PD-1 and/or 2B4. Percentage distribution in each quadrant is displayed (PD-1 vs 2B4), with the boxed number denoting percentage of cells coexpressing all 3 markers.