LacZ staining of whole-mount organs from vWF^LacZ/+ and vWFΔInt^LacZ/+ mice. Hearts, chest walls, en face aortae, brains, and diaphragms were harvested from 6- to 8-week-old male (A,C,E,G,H) vWF^LacZ/+ mice and (B,D,F,G,H) vWFΔInt^LacZ/+ mice and processed in parallel for whole-mount staining with X-Gal. (G) Brain from the vWF^LacZ/+ mouse is on the left, and the brain from the vWFΔInt^LacZ/+ mouse is on the right. (H) Diaphragm from the vWF^LacZ/+ mouse is on the left, and the diaphragm from the vWFΔInt^LacZ/+ mouse is on the right. Whole-mount tissues were analyzed under a Nikon SMZ-U dissecting microscope, and microphotographs were collected using a Nikon Coolpix 8400 camera.