LacZ staining of whole-mount organs and bone marrow from vWF-LacZ knockin mice with or without native or heterologous intron. (A-E) Hearts, (F-J) diaphragms, (K-O) chest walls, (P-T) brains, and (U-Y) en face aortae were harvested from 6- to 8-week-old male vWF^LacZ/+, vWFΔInt^LacZ/+, vWFGIn^LacZ/+, vWFDIn^LacZ/+, and vWFHIn^LacZ/+ mice and processed in parallel for whole-mount staining with X-Gal. The chest wall shows the intercostal space between two ribs (a superior rib on the left and an inferior rib on the right). (Z,Aa-Ad) Bone marrow aspirates were stained for LacZ. Whole-mount tissues were analyzed under a Nikon SMZ-U dissecting microscope, and microphotographs were collected using a Nikon Coolpix 8400 camera. Scale bar, 200 μM.